Gatlin finished third in his semi in 20.13 seconds, leaving him ninth overall.
Bolt stayed on track for a historic sprint triple by clocking the fastest time of 19.78.
He has already pocketed a third Olympic 100m title in Rio and will also go for gold in the 4x100m relay later in the Games.
Fellow Jamaican sprinter Elaine Thompson today added a women’s 200m gold to her 100m title at the Rio Olympics.
Thompson finished in 21.78 seconds – the fastest time in the world this year – to edge Dutch rival Dafne Schippers, who crossed in 21.88 before sprawling across the ground in exhaustion. American Tori Bowie took bronze in 22.15.
Also in athletics, Australian Olympic debutante Brooke Stratton finished seventh in the women’s long jump. Stratton, 26, produced her best jump of 6.74m in the fourth round.
Tianna Bartoletta from the United States claimed the gold medal with 7.17m ahead of fellow American Brittney Reese and Serbia’s Ivana Spanovic.
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